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Article

[Cloning of Blakeslea trispora carRA gene by PCR-driven overlap extension and construction of an activity detection system].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Jul 2011

Tang Hui, Shi Nan, Yu Miao, Liu Long, Liu Jing, Jia Ying, Niu Hongyan, Zhang Liping

Abstract excerpt

Blakeslea trispora CarRA has both lycopene cyclase and phytoene synthase activity. In order to analyze the double functional activity of CarRA proteins and to detect the active sites of lycopene cyclase, we constructed two detection systems in Escherichia coli by color complementary. Through PCR-driven overlap extension we got carRA gene cDNA, then constructed prokaryotes expression vector pET28a-carRA....

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PMID: 22016982

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