Article
[Preparing of a ssDNA target in a single-round PCR with low-melt excess primer for hybridization with microarrays].
Molekuliarnaia biologiia - 1 Jan 2000
Fesenko D O, Korienko A E, Chudinov A V, Nasedkina T V
Abstract excerpt
Method of ssDNA preparing in single-round PCR for microarray application is described. The approach is exemplified on genotyping of DARC gene. It is opposed to two-round PCR that consists of separate symmethric and asymmethric stages. Implementation of reaction in single round is achieved by means of low-melt excess internal primer application. The primer do not anneal during symmethric stage but after decreasing...
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