Article
Isolation and molecular characterization of spontaneously occurring cytolysin-negative mutants of Actinobacillus pleuropneumoniae serotype 7.
Infection and immunity - 1 Nov 1991
Anderson C, Potter A A, Gerlach G F
Abstract excerpt
Actinobacillus pleuropneumoniae serotype 7 strains are shown to spontaneously lose cytolytic activity with a frequency of approximately 10(-4). The phenotypic change is associated with the loss of approximately 8.5 kbp of chromosomal DNA. A genomic fragment encoding the cytolysin and its flanking...
Topics
- Actinobacillus pleuropneumoniae
- Base Sequence
- Blotting, Southern
- Chromosome Deletion
- Cloning, Molecular
- Cytotoxins
- DNA Mutational Analysis
- DNA, Bacterial
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Repetitive Sequences, Nucleic Acid
- Restriction Mapping