Article
N-myristoylation is required for function of the pheromone-responsive G alpha protein of yeast: conditional activation of the pheromone response by a temperature-sensitive N-myristoyl transferase.
Genes & development - 1 Nov 1991
Stone D E, Cole G M, de Barros Lopes M, Goebl M, Reed S I
Abstract excerpt
In a screen designed to identify novel mutations in the mating response pathway of Saccharomyces cerevisiae, we isolated conditional alleles of NMT1, the gene encoding N-myristoyl transferase. Genetic data indicate that Nmt1 deficiency results in the activation of the pheromone response at the level of Gpa1, the alpha subunit of the pheromone-responsive G protein. We show that Gpa1 is myristoylated by Nmt1, and...
Topics
- Acyltransferases
- Base Sequence
- Blotting, Western
- GTP-Binding Proteins
- Genes, Fungal
- Kinetics
- Mating Factor
- Molecular Sequence Data
- Mutation
- Myristic Acid