Article
Regulation of Vif mRNA splicing by human immunodeficiency virus type 1 requires 5' splice site D2 and an exonic splicing enhancer to counteract cellular restriction factor APOBEC3G.
Journal of virology - 1 Jun 2009
Mandal Dibyakanti, Exline Colin M, Feng Zehua, Stoltzfus C Martin
Abstract excerpt
The human immunodeficiency virus type 1 (HIV-1) accessory protein Vif is encoded by an incompletely spliced mRNA resulting from splicing of the major splice donor in the HIV-1 genome, 5' splice site (5'ss) D1, to the first splice acceptor, 3'ss A1. We have shown previously that splicing of HIV-1 vif mRNA is tightly regulated by suboptimal 5'ss D2, which is 50 nucleotides downstream of 3'ss A1; a GGGG silencer...
Topics
- APOBEC-3G Deaminase
- Cell Line
- Cytidine Deaminase
- Gene Expression Regulation, Viral
- HIV-1
- Humans
- Mutation
- RNA Splice Sites
- RNA Splicing
- RNA, Viral
- Virus Replication
- vif Gene Products, Human Immunodeficiency Virus