Article
Helices alpha2 and alpha3 of West Nile virus capsid protein are dispensable for assembly of infectious virions.
Journal of virology - 1 Jun 2009
Schlick Petra, Taucher Christian, Schittl Beate, Tran Janina L, Kofler Regina M, Schueler Wolfgang, von Gabain Alexander, Meinke Andreas, Mandl Christian W
Abstract excerpt
The internal hydrophobic sequence within the flaviviral capsid protein (protein C) plays an important role in the assembly of infectious virions. Here, this sequence was analyzed in a West Nile virus lineage I isolate (crow V76/1). An infectious cDNA clone was constructed and used to introduce deletions into the internal hydrophobic domain which comprises helix alpha2 and part of the loop intervening helices...
Topics
- Amino Acid Sequence
- Animals
- Capsid Proteins
- Cell Line
- Cell Proliferation
- Chlorocebus aethiops
- Cricetinae
- DNA, Complementary
- Gene Deletion
- Genome, Viral
- Models, Molecular
- Molecular Sequence Data
- Mutation