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Comparison of 7 Methods for Extracting Cell-Free DNA from Serum Samples of Colorectal Cancer Patients

25 Feb 2009

Abstract excerpt

The presence of cell-free DNA of tumor origin in serum or plasma of cancer patients (1) has triggered numerous studies to explore the diagnostic and prognostic potential of circulating DNA. This DNA, present only in minute concentrations in plasma or serum, is highly fragmented (2), a condition that often leads to substantial loss of DNA of small sizes in the course of DNA isolation. The lack of consensus...

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PMID: 19246404
DOI: 10.1373/clinchem.2008.110122

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