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Improved Method for Isolating Cell-Free DNA

22 Jul 2005

Abstract excerpt

After Mandel and Metais (1) had originally described the existence of free circulating nucleic acids in plasma/serum in humans, Stroun et al. (2)(3) demonstrated that part of the free circulating DNA in cancer patients is of tumor origin (4)(5)(6). The quantity of DNA that can be isolated from human plasma/serum is very low and is frequently the limiting factor when a larger marker panel is to be used for genetic...

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PMID: 16040863
DOI: 10.1373/clinchem.2005.051003

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