Article
Importance of the phenotypic state of vascular smooth muscle cells on the binding and the mitogenic activity of endothelin.
Peptides - 1 Jan 2000
Serradeil-Le Gal C, Herbert J M, Garcia C, Boutin M, Maffrand J P
Abstract excerpt
Smooth muscle cells of the rabbit aorta, when grown in vitro, express distinguishable forms of phenotypes (contractile and synthetic). On contractile cells, ET-1 specifically bound to a single class of high affinity (KD = 128 pM) and high capacity (Bmax = 66,000 sites/cell) binding sites. But, whereas affinity of [125I]-ET-1 was not significantly affected by phenotypic modulation, synthetic cells displayed a...
Topics
- Animals
- Cell Division
- Cells, Cultured
- Endothelins
- Mitogens
- Muscle Contraction
- Muscle, Smooth, Vascular
- Phenotype