Article
A novel assay for rapid in vivo determination of phenotypic stability of recombinant ethanol-producing microorganisms.
Bioresource technology - 1 Jun 2009
Zhou Bin, Martin Gregory J O, Pamment Neville B
Abstract excerpt
A rapid empirical assay is presented for assessing the phenotypic stability of continuous cultures of recombinant bacteria containing transposed pdc and adh genes for ethanol production. The method measures spectrophotometrically the rate of colour formation when cells oxidize added ethanol to acetaldehyde in the presence of Schiff's reagent. During chemostat cultures of the recombinant ethanologen Escherichia...
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