Article
Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae.
Molecular and cellular biology - 1 Nov 1991
Malone E A, Clark C D, Chiang A, Winston F
Abstract excerpt
SPT16 was previously identified as a high-copy-number suppressor of delta insertion mutations in the 5' regions of the HIS4 and LYS2 genes of Saccharomyces cerevisiae. We have constructed null mutations in the SPT16 gene and have demonstrated that it is essential for growth. Temperature-sensitive-lethality spt16 alleles have been isolated and shown to be pleiotropic; at a temperature permissive for growth, spt16...
Topics
- DNA, Fungal
- Genes, Fungal
- Genotype
- Mutagenesis, Insertional
- Plasmids
- Promoter Regions, Genetic
- Restriction Mapping
- Saccharomyces cerevisiae
- Suppression, Genetic
- Transcription, Genetic