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Human Embryos Derived by Somatic Cell Nuclear Transfer Using an Alternative Enucleation Approach

23 Jan 2009

Abstract excerpt

Somatic cell nuclear transfer (SCNT) was used to generate patient-specific embryonic stem cells (ESCs) from blastocysts cloned by nuclear transfer (ntESCs). In this study, a total of 135 oocytes were obtained from 12 healthy donors (30-35 years). Human oocytes, obtained within 2 h following transvaginal aspiration, were enucleated using a Spindle Imaging System to position the spindle and chromosomes that lay on...

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PMID: 19196043
DOI: 10.1089/clo.2008.0041

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