Article
Reprogramming of human somatic cells using human and animal oocytes.
Cloning and stem cells - 1 Jun 2009
Chung Young, Bishop Colin E, Treff Nathan R, Walker Stephen J, Sandler Vladislav M, Becker Sandy, Klimanskaya Irina, Wun Wan-Song, Dunn Randall, Hall Rebecca M, Su Jing, Lu Shi-Jiang, Maserati Marc, Choi Young-Ho, Scott Richard, Atala Anthony, Dittman Ralph, Lanza Robert
Abstract excerpt
There is renewed interest in using animal oocytes to reprogram human somatic cells. Here we compare the reprogramming of human somatic nuclei using oocytes obtained from animal and human sources. Comparative analysis of gene expression in morula-stage embryos was carried out using single-embryo transcriptome amplification and global gene expression analyses. Genomic DNA fingerprinting and PCR analysis confirmed...
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