Article
Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer's disease-causing dominant mutation APPsw.
Transgenic research - 1 Aug 2009
Kragh Peter M, Nielsen Anders Lade, Li Juan, Du Yutao, Lin Lin, Schmidt Mette, Bøgh Ingrid Brück, Holm Ida E, Jakobsen Jannik E, Johansen Marianne G, Purup Stig, Bolund Lars, Vajta Gábor, Jørgensen Arne Lund
Abstract excerpt
In an effort to develop a porcine model of Alzheimer's disease we used handmade cloning to produce seven transgenic Göttingen minipigs. The donor fibroblasts had been stably transfected with a plasmid cassette containing, as transgene, the cDNA of the neuronal variant of the human amyloid precursor protein gene with the Swedish mutation preceded by beta-globin sequences to induce splicing and a human PDGF beta...
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