Article
Ectopic (illegitimate) transcription: new possibilities for the analysis and diagnosis of human genetic disease.
Annals of medicine - 1 Feb 1994
Cooper D N, Berg L P, Kakkar V V, Reiss J
Abstract excerpt
By means of the Polymerase Chain Reaction (PCR), 'ectopic' or 'illegitimate' transcripts from any gene may be amplified from any tissue or cell type. RNA transcript analysis is therefore no longer dependent upon possession of the often inaccessible 'expressing' tissue. We review here the applications of ectopic transcript analysis to mutation detection and characterization, analysis of RNA splicing and the study...
Topics
- Genetic Diseases, Inborn
- Genotype
- Humans
- Mutation
- Phenotype
- Polymerase Chain Reaction
- RNA Splicing
- Transcription, Genetic