Article
Are the histidine residues of glutathione S-transferase important in catalysis? An assessment by 13C NMR spectroscopy and site-specific mutagenesis.
The Journal of biological chemistry - 15 Oct 1991
Zhang P H, Graminski G F, Armstrong R N
Abstract excerpt
To test the proposition that a histidine residue is essential in the catalytic mechanism of glutathione S-transferase, rat liver isoenzyme 3-3 specifically labeled with [ring-2-13C]histidine was prepared. The 13C NMR spectrum of the labeled enzyme revealed four resonances corresponding to the 4 histidine residues in the mu gene class type 3 subunit. Titration of the four resonances in the range of pH 4-9 both in...
Topics
- Animals
- Catalysis
- Escherichia coli
- Gene Expression
- Glutathione Transferase
- Histidine
- Hydrogen-Ion Concentration
- Isoenzymes
- Kinetics
- Liver
- Magnetic Resonance Spectroscopy