Article
Role of cysteine residues in human NADH-cytochrome b5 reductase studied by site-directed mutagenesis. Cys-273 and Cys-283 are located close to the NADH-binding site but are not catalytically essential.
The Journal of biological chemistry - 25 Apr 1991
Shirabe K, Yubisui T, Nishino T, Takeshita M
Abstract excerpt
Human NADH-cytochrome b5 reductase (EC 1.6.2.2) contains 4 cyteine residues (Cys-203, -273, -283, and -297). Cys-283 was previously proposed to be involved in NADH binding by chemical modification (Hackett, C. S., Novoa, W. B., Ozols, J., and Strittmatter, P. (1986) J. Biol. Chem. 261, 9854-9857). In the present study the role of cysteines in the enzyme was probed by replacing these residues by Ser, Ala, or Gly...
Topics
- Base Sequence
- Binding Sites
- Cysteine
- Cytochrome Reductases
- Cytochrome-B(5) Reductase
- Humans
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation