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Article

A structural role of histidine 15 in human glutathione transferase M1-1, an amino acid residue conserved in class Mu enzymes.

Protein engineering - 1 Sept 1992

Widersten M, Mannervik B

Abstract excerpt

His15 is a conserved amino acid residue in all known class Mu glutathione transferases. This His residue in human glutathione transferase M1-1 has been mutated into 17 different amino acid residues by means of site-directed random mutagenesis to determine if any substitutions are compatible with catalytic activity. The majority of the mutant proteins appeared unstable and could not be isolated in reasonable...

Topics

Alleles
Amino Acid Sequence
Base Sequence
Chemical Phenomena
Chemistry, Physical
Glutathione Transferase
Histidine
Humans
Kinetics
Molecular Sequence Data
Mutagenesis, Site-Directed

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Recombinant Fusion Proteins

Source

Identifiers

PMID: 1438166
DOI: 10.1093/protein/5.6.551

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1438166-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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