Article
Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16.
Journal of bacteriology - 1 Oct 1990
Dahler G S, Barras F, Keen N T
Abstract excerpt
A 14-kilobase BamHI-EcoRI DNA fragment cloned from Erwinia chrysanthemi EC16 contained a gene encoding a metalloprotease inhibitor as well as three tandem prt genes encoding metalloproteases. The prt genes were separated from the inhibitor gene by a ca. 4-kilobase region that was necessary for extracellular secretion of the proteases. When individually subcloned downstream from vector promoters, the three prt...
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