Article
Human platelet antigen typing of neonatal alloimmune thrombocytopenia patients using whole genome amplified DNA and a 5'-nuclease assay.
Transfusion - 1 May 2009
Lemnrau Alina G, Cardoso Sandra, Creary Lisa E, Brown Colin, Miretti Marcos, Girdlestone John, Navarrete Cristina V
Abstract excerpt
BACKGROUND: A serious constraint in the investigation of the human platelet antigen (HPA) status of potential neonatal alloimmune thrombocytopenia (NAIT) cases is the limited amount of DNA available from the neonates. Whole genome amplification (WGA) of these DNA samples could overcome this problem, but requires validation to ensure that it is sufficiently sensitive and accurate before its application in a...
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