Article
Geminivirus-based shuttle vectors capable of replication in Escherichia coli and monocotyledonous plant cells.
Gene - 15 Aug 1991
Kammann M, Matzeit V, Schmidt B, Schell J, Walden R, Gronenborn B
Abstract excerpt
Shuttle vectors have been constructed that are able to replicate in either Escherichia coli or plant cells. They contain the ColE1 origin of replication and parts of the wheat dwarf virus genome, a geminivirus infecting a variety of species of monocotyledonous plants. Such plasmids are able to replicate in E. coli and wheat cells. The plasmids can be rescued in E. coli and show no changes during their passage...
Topics
- Bacteriocin Plasmids
- Cloning, Molecular
- Escherichia coli
- Genetic Vectors
- Mutation
- Plant Viruses
- Triticum
- Virus Replication
