Article
In planta cloning of geminiviral DNA: the true Sida micrantha mosaic virus.
Journal of virological methods - 1 Feb 2010
Jeske Holger, Gotthardt Diether, Kober Sigrid
Abstract excerpt
The circular single-stranded DNAs of geminiviruses are multiplied efficiently and preferentially by rolling circle amplification (RCA), and can be diagnosed readily by restriction fragment length polymorphism (RFLP) and direct sequencing of the RCA product. Two strategies are described for cloning geminiviruses from plants harboring mixed infections by using RCA and RFLP with plant-derived nucleic acids without...
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