Article
Development of tobacco ringspot virus-based vectors for foreign gene expression and virus-induced gene silencing in a variety of plants.
Virology - 1 May 2016
Zhao Fumei, Lim Seungmo, Igori Davaajargal, Yoo Ran Hee, Kwon Suk-Yoon, Moon Jae Sun
Abstract excerpt
We report here the development of tobacco ringspot virus (TRSV)-based vectors for the transient expression of foreign genes and for the analysis of endogenous gene function in plants using virus-induced gene silencing. The jellyfish green fluorescent protein (GFP) gene was inserted between the TRSV movement protein (MP) and coat protein (CP) regions, resulting in high in-frame expression of the RNA2-encoded viral...
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