Article
An Improved Brome mosaic virus Silencing Vector: Greater Insert Stability and More Extensive VIGS.
Plant physiology - 1 Jan 2018
Ding Xin Shun, Mannas Stephen W, Bishop Bethany A, Rao Xiaolan, Lecoultre Mitchell, Kwon Soonil, Nelson Richard S
Abstract excerpt
Virus-induced gene silencing (VIGS) is used extensively for gene function studies in plants. VIGS is inexpensive and rapid compared with silencing conducted through stable transformation, but many virus-silencing vectors, especially in grasses, induce only transient silencing phenotypes. A major reason for transient phenotypes is the instability of the foreign gene fragment (insert) in the vector during VIGS....
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