Article
Assays for determining poly(A) tail length and the polarity of mRNA decay in mammalian cells.
Methods in enzymology - 1 Jan 2008
Murray Elizabeth L, Schoenberg Daniel R
Abstract excerpt
This chapter describes several methods for measuring the length of the mRNA poly(A) tail and a novel method for measuring mRNA decay. Three methods for measuring the length of a poly(A) tail are presented: the poly(A) length assay, the ligation-mediated poly(A) test (LM-PAT), and the RNase H assa...
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