Cloning and DNA sequence of amiC, a new gene regulating expression of the Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC product.

Abstract excerpt

Using in vitro-constructed deletions and subcloned DNA fragments, we have identified a new gene, amiC, which regulates expression of the inducible Pseudomonas aeruginosa aliphatic amidase activity. The DNA sequence of the gene has been determined, and an open reading frame encoding a polypeptide of 385 amino acids (molecular mass, 42,834 Da) has been identified. A search of sequence libraries has failed to find...

Topics

• Amidohydrolases
• Amino Acid Sequence
• Bacterial Proteins
• Base Sequence
• Enzyme Induction
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Genes, Regulator
• Molecular Sequence Data
• Mutation
• Periplasmic Binding Proteins