Identification and characterization of aarF, a locus required for production of ubiquinone in Providencia stuartii and Escherichia coli and for expression of 2'-N-acetyltransferase in P. stuartii.

Abstract excerpt

Providencia stuartii contains a chromosomal 2'-N-acetyltransferase [AAC(2')-Ia] involved in the O acetylation of peptidoglycan. The AAC(2')-Ia enzyme is also capable of acetylating and inactivating certain aminoglycosides and confers high-level resistance to these antibiotics when overexpressed. We report the identification of a locus in P. stuartii, designated aarF, that is required for the expression of...

Topics

• Acetyltransferases
• Amino Acid Sequence
• Bacterial Proteins
• Cloning, Molecular
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Genes, Bacterial
• Genetic Complementation Test
• Models, Chemical
• Models, Genetic
• Molecular Sequence Data
• Mutation