Article
Replacement of an electron transfer pathway in cytochrome c peroxidase with a surrogate peptide.
Biochemistry - 13 Jan 2009
Hays Putnam Anna-Maria A, Lee Young-Tae, Goodin David B
Abstract excerpt
A proposed electron transfer pathway in cytochrome c peroxidase was previously excised from the structure by design. The engineered channel mutant was shown to bind peptide surrogates without restoration of cyt c oxidation. Here, we report the 1.6 A crystal structure of (N-benzimidazole-propionic acid)-Gly-Ala-Ala bound within the engineered channel. The peptide retains many features of the native electron...
Topics
- Benzimidazoles
- Crystallography, X-Ray
- Cytochrome-c Peroxidase
- Electron Transport
- Models, Molecular
- Mutation
- Oligopeptides
- Oxidation-Reduction
- Protein Binding
- Protein Conformation