Article
In vitro assembly of the Tomato bushy stunt virus replicase requires the host Heat shock protein 70.
Proceedings of the National Academy of Sciences of the United States of America - 16 Dec 2008
Pogany Judit, Stork Jozsef, Li Zhenghe, Nagy Peter D
Abstract excerpt
To gain insights into the functions of a viral RNA replicase, we have assembled in vitro and entirely from nonplant sources, a fully functional replicase complex of Tomato bushy stunt virus (TBSV). The formation of the TBSV replicase required two purified recombinant TBSV replication proteins, which were obtained from E. coli, the viral RNA replicon, rATP, rGTP, and a yeast cell-free extract. The in vitro...
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