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Article

Design and expression of human alpha7 nicotinic acetylcholine receptor extracellular domain mutants with enhanced solubility and ligand-binding properties.

Biochimica et biophysica acta - 1 Feb 2009

Zouridakis Marios, Zisimopoulou Paraskevi, Eliopoulos Elias, Poulas Konstantinos, Tzartos Socrates J

Abstract excerpt

In order to facilitate structural studies of the extracellular domain (ECD) of human alpha7 nicotinic acetylcholine receptor (nAChR), we designed several mutants, since the wild-type-ECD forms large oligomers and microaggregates, and expressed them in the yeast Pichia pastoris. Mutant design was based on a 3D model of human alpha7-nAChR-ECD, constructed using as templates the X-ray crystal structure of the...

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PMID: 19059502
DOI: 10.1016/j.bbapap.2008.11.002

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ECD

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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