Article
Relative contributions of MCM1 and STE12 to transcriptional activation of a- and alpha-specific genes from Saccharomyces cerevisiae.
Molecular & general genetics : MGG - 1 Jun 1991
Hwang-Shum J J, Hagen D C, Jarvis E E, Westby C A, Sprague G F
Abstract excerpt
We have examined the relative contributions of MCM1 and STE12 to the transcription of the a-specific STE2 gene by using a 367 bp fragment from the STE2 5'-noncoding region to drive expression of a reporter lacZ gene. Mutation of the MCM1 binding site destroyed MCM1.alpha 2-mediated repression in alpha cells and dramatically reduced expression in a cells. The residual expression was highly stimulated by exposure...
Topics
- Base Sequence
- DNA-Binding Proteins
- Enhancer Elements, Genetic
- Fungal Proteins
- Genes, Fungal
- Genes, Regulator
- Mating Factor
- Molecular Sequence Data
- Mutation
- Peptides
- Pheromones