Biosynthesis of the cloned intestinal Na+/glucose cotransporter.

doi:10.1016/0005-2736(91)90323-z

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Biosynthesis of the cloned intestinal Na+/glucose cotransporter.

Biochimica et biophysica acta - 7 May 1991

Hediger M A, Mendlein J, Lee H S, Wright E M

Abstract excerpt

The initial stages in the biosynthesis of the cloned Na+/glucose cotransporter were examined by in vitro expression of the protein in the absence and presence of pancreatic microsomes. Glycosylation was detected by endoglycosidase-H shifts in the apparent size of the proteins on SDS-PAGE. In the presence of microsomes, Mr increased from 52,000 to 58,000, and this was reversed by endo-H. This demonstrates that the...

Topics

Acetylglucosaminidase
Animals
Base Sequence
Cells, Cultured
Cloning, Molecular
DNA
Gene Expression
Glycosylation
Intestinal Mucosa
Intestines
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Molecular Weight

Monosaccharide Transport Proteins

Mutation

Oocytes

Protein Conformation

Rabbits

Source

Open in PubMed

Identifiers

PMID: 1903656
DOI: 10.1016/0005-2736(91)90323-z

Genes

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1903656-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 8 closest

Biosynthesis of the cloned intestinal Na+/glucose cotransporter.PMID 1903656Read ↗

Glycans in the intestinal peptide transporter PEPT1 contribute to function and protect from proteolysis.Semantic proximity Read ↗

Functional studies of the rabbit intestinal Na+/glucose carrier (SGLT1) expressed in COS-7 cells: evaluation of the mutant A166C indicates this region is important for Na+-activation of the carrier.Semantic proximity Read ↗

Characterization of Na,K-ATPase and H,K-ATPase enzymes with glycosylation-deficient beta-subunit variants by voltage-clamp fluorometry in Xenopus oocytes.Semantic proximity Read ↗

The putative amino-terminal signal peptide of the cloned rat brain Na(+)-Ca2+ exchanger gene (RBE-1) is not mandatory for functional expression.Semantic proximity Read ↗

Glycosylation of shaker potassium channel protein in insect cell culture and in Xenopus oocytes.Semantic proximity Read ↗

Identification of an internal topogenic signal sequence in human Band 3, the erythrocyte anion exchanger.Semantic proximity Read ↗

Polarized distribution of glucose transporter isoforms in Caco-2 cells.Semantic proximity Read ↗

[Structure and function of glucose transporters].Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.