Article
Biosynthesis of the cloned intestinal Na+/glucose cotransporter.
Biochimica et biophysica acta - 7 May 1991
Hediger M A, Mendlein J, Lee H S, Wright E M
Abstract excerpt
The initial stages in the biosynthesis of the cloned Na+/glucose cotransporter were examined by in vitro expression of the protein in the absence and presence of pancreatic microsomes. Glycosylation was detected by endoglycosidase-H shifts in the apparent size of the proteins on SDS-PAGE. In the presence of microsomes, Mr increased from 52,000 to 58,000, and this was reversed by endo-H. This demonstrates that the...
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