Article
Functional studies of the rabbit intestinal Na+/glucose carrier (SGLT1) expressed in COS-7 cells: evaluation of the mutant A166C indicates this region is important for Na+-activation of the carrier.
The Biochemical journal - 15 May 1998
Vayro S, Lo B, Silverman M
Abstract excerpt
We have exploited two mutants of the rabbit intestinal Na+/glucose carrier SGLT1 to explore the structure/function relationship of this Na+/glucose transporter in COS-7 cells. A functional N-terminal myc-epitope-tagged SGLT1 protein was constructed and used to determine the plasma-membrane locali...
Topics
- Animals
- Biological Transport
- COS Cells
- Cysteine
- Intestines
- Kinetics
- Membrane Glycoproteins
- Methylglucosides
- Microscopy, Immunoelectron
- Monosaccharide Transport Proteins
- Mutation
- Phlorhizin
- Rabbits
- Sodium
- Sodium-Glucose Transporter 1
- Structure-Activity Relationship
- Substrate Specificity
- Transfection