Article
A natural prothrombin mutant reveals an unexpected influence of A-chain structure on the activity of human alpha-thrombin.
The Journal of biological chemistry - 26 Mar 2004
De Cristofaro Raimondo, Akhavan Sepideh, Altomare Cosimo, Carotti Andrea, Peyvandi Flora, Mannucci Pier Mannuccio
Abstract excerpt
We have recently identified in two unrelated patients with bleeding tendency a homozygous mutation causing a deletion of one of the two contiguous Lys(9)/Lys(10) residues in the A-chain of alpha-thrombin (DeltaK9). We used in vitro expression analysis to clarify the role of the deletion of Lys(9) or Lys(10) in the thrombin function. The k(cat)/K(m) value of the hydrolysis by DeltaK9 of the synthetic substrate...
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