Article
Sequence diversity of the Bla g 4 cockroach allergen, homologous to lipocalins, from Blattella germanica.
International archives of allergy and immunology - 1 Jan 2009
Jeong Kyoung Yong, Yi Myung-Hee, Jeong Kyoung-jin, Lee Haeseok, Hong Chein-Soo, Yong Tai-Soon
Abstract excerpt
BACKGROUND: The cockroach allergen Bla g 4, a putative lipocalin, is known to exhibit frequent sequence variations. However, the previously reported cDNA sequences are truncated at the N terminus. This study was undertaken to investigate the mechanisms by which these sequence variations are generated. METHODS: Rapid amplification of cDNA ends PCR and RT-PCR were performed to obtain the full sequence of the Bla g...
Topics
- Allergens
- Amino Acid Sequence
- Animals
- Antibodies
- Antigens, Plant
- Blattellidae
- Cloning, Molecular
- DNA, Complementary
- Female
- Genetic Variation
- Insect Proteins
- Lipocalins
- Male
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Sequence Homology, Amino Acid