Article
Isolation and Characterization of Two Complete Ara h 2 Isoforms cDNA
1 Jan 2003
Abstract excerpt
BACKGROUND: Ara h 2 is a major peanut allergen recognized by IgE in more than 90% of patients. After electrophoretic separation the purified protein exists as a doublet, and sequences of one incomplete cDNA and one genomic clone for this allergen have been reported. METHODS: Ara h 2 isoforms were purified and analyzed by mass spectroscopy, and PCR amplification products of Ara h 2 were cloned and sequenced....
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