Article
Demonstration of the interaction of transforming growth factor beta 2 and type X collagen using a modified tandem affinity purification tag.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 15 Nov 2008
Yang Maozhou, Wang Xinli, Zhang Liang, Yu Chiyang, Zhang Bingbing, Cole William, Cavey Greg, Davidson Paula, Gibson Gary
Abstract excerpt
Like other members of the transforming growth factor beta (TGF-beta) family of growth factors, the biological activity of TGF-beta2 is believed to be regulated by the formation and dissociation of multiprotein complexes. To isolate the molecular complex formed by TGF-beta2 secreted by hypertrophic chondrocytes we have used expression of TGF-beta2 fused with the humanized, tandem affinity purification (hTAP) tag...
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