Article
Differences in dynamic structure of LC8 monomer, dimer, and dimer-peptide complexes.
Biochemistry - 18 Nov 2008
Hall Justin, Hall Andrea, Pursifull Nathan, Barbar Elisar
Abstract excerpt
Dimerization of dynein light chain LC8 creates two symmetric grooves at the dimer interface with diverse binding capabilities. In addition to pH and protein concentration, dimerization is affected by phosphorylation, as illustrated by a phosphomimetic mutation that promotes dissociation of LC8 to a monomer and subsequent dissociation from the dynein complex in vitro. In this work we characterize the dynamic...
Topics
- Animals
- Carrier Proteins
- Dimerization
- Drosophila Proteins
- Drosophila melanogaster
- Dyneins
- Hydrogen-Ion Concentration
- Ligands
- Models, Molecular
- Mutation
- Peptides
- Protein Binding
- Protein Structure, Quaternary
- RNA-Binding Proteins