Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Replacement of the axial histidine heme ligand with cysteine in nitrophorin 1: spectroscopic and crystallographic characterization.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry - 1 Feb 2009

Vetter Stefan W, Terentis Andrew C, Osborne Robert L, Dawson John H, Goodin David B

Abstract excerpt

To evaluate the potential of using heme-containing lipocalin nitrophorin 1 (NP1) as a template for protein engineering, we have replaced the native axial heme-coordinating histidine residue with glycine, alanine, and cysteine. We report here the characterization of the cysteine mutant H60C_NP1 by spectroscopic and crystallographic methods. The UV/vis, resonance Raman, and magnetic circular dichroism spectra...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Circular Dichroism
Share to Heme

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hemeproteins

Share to Histidine

Share to Protein Engineering

Share to Salivary Proteins and Peptides

Share to Spectrophotometry, Ultraviolet

Share to Spectrum Analysis, Raman

Source

Open in PubMed Open full text

Identifiers

PMID: 18923851
DOI: 10.1007/s00775-008-0436-x
PMCID: PMC2635096

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Replacement of the axial histidine heme ligand with cysteine in nitrophorin 1: spectroscopic and crystallographic characterization.PMID 18923851Read ↗

Distal-to-proximal NO conversion in hemoproteins: the role of the proximal pocket.Semantic proximity Read ↗

Investigation of Cyanide Ligand as an Active Site Probe of Human Heme Oxygenase.Semantic proximity Read ↗

Structural determinants of factor IX(a) binding in nitrophorin 2, a lipocalin inhibitor of the intrinsic coagulation pathway.Semantic proximity Read ↗

Structural insights into the role of iron-histidine bond cleavage in nitric oxide-induced activation of H-NOX gas sensor proteins.Semantic proximity Read ↗

Alkylamine-ligated H93G myoglobin cavity mutant: a model system for endogenous lysine and terminal amine ligation in heme proteins such as nitrite reductase and cytochrome f.Semantic proximity Read ↗

Exploring second coordination sphere effects in nitric oxide synthase.Semantic proximity Read ↗

Structural characterization of nitrosomonas europaea cytochrome c-552 variants with marked differences in electronic structure.Semantic proximity Read ↗

Ni(II)-binding affinity of CcNikZ-II and its homologs: the role of the HH-prong and variable loop revealed by structural and mutational studies.Semantic proximity Read ↗

Role of binding site loops in controlling nitric oxide release: structure and kinetics of mutant forms of nitrophorin 4.Semantic proximity Read ↗

Crystal Structure and Conformational Change Mechanism of a Bacterial Nramp-Family Divalent Metal Transporter.Semantic proximity Read ↗

Redox-dependent stability, protonation, and reactivity of cysteine-bound heme proteins.Semantic proximity Read ↗

Effect of Outer-Sphere Side Chain Substitutions on the Fate of the trans Iron-Nitrosyl Dimer in Heme/Nonheme Engineered Myoglobins (Fe(B)Mbs): Insights into the Mechanism of Denitrifying NO Reductases.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.