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Article

Simple approach to reduce PCR artefact formation leads to reliable genotyping of MHC and other highly polymorphic loci--implications for evolutionary analysis.

Gene - 31 Dec 2008

Lenz Tobias L, Becker Sven

Abstract excerpt

Genetic variation in coding regions is of strong interest for biologists as it represents an important factor that drives evolution. To analyse polymorphic loci, researchers usually rely on commonly used typing techniques such as cloning, SSCP, DGGE or RSCA. However, there are potential pitfalls...

Read the complete abstract on PubMed

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Share to Cloning, Molecular
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Major Histocompatibility Complex

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Share to Polymorphism, Single-Stranded Conformational

Share to Sequence Analysis, DNA

Share to Sequence Homology, Nucleic Acid

Source

Identifiers

PMID: 18848974
DOI: 10.1016/j.gene.2008.09.013

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-18848974-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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