Article
1.59 A structure of trypsin at 120 K: comparison of low temperature and room temperature structures.
Proteins - 1 Jan 1991
Earnest T, Fauman E, Craik C S, Stroud R
Abstract excerpt
The structure of a rat trypsin mutant [S195C] at a temperature of 120 K has been refined to a crystallographic R factor of 17.4% between 12.0 and 1.59 A and is compared with the structure of the D102N mutant at 295 K. A reduction in the unit cell dimensions in going from room temperature to low temperature is accompanied by a decrease in molecular surface area and radius of gyration. The overall structure remains...
Topics
- Animals
- Cold Temperature
- Models, Molecular
- Molecular Structure
- Mutation
- Protein Conformation
- Rats
- Temperature
- Trypsin
- X-Ray Diffraction