Article
Atomic resolution structure of squash trypsin inhibitor: unexpected metal coordination.
Acta crystallographica. Section D, Biological crystallography - 1 Sept 2002
Thaimattam Ram, Tykarska Ewa, Bierzynski Andrzej, Sheldrick George M, Jaskolski Mariusz
Abstract excerpt
CMTI-I, a small-protein trypsin inhibitor, has been crystallized as a 4:1 protein-zinc complex. The metal is coordinated in a symmetric tetrahedral fashion by glutamate/glutamic acid side chains. The structure was solved by direct methods in the absence of prior knowledge of the special position metal centre and refined with anisotropic displacement parameters using diffraction data extending to 1.03 A. In the...
Topics
- Crystallography, X-Ray
- Cucurbita
- Hydrogen Bonding
- Metals
- Models, Molecular
- Mutation
- Plant Proteins
- Protein Conformation