Role of the aromatic residues in the near-amino terminal motif of vimentin in intermediate filament assembly in vitro.

Abstract excerpt

Type III and IV intermediate filament (IF) proteins share a conserved sequence motif of -Tyr-Arg-Arg-X-Phe- at the near-amino termini. To characterize significance of the aromatic residues in the motif, we prepared vimentin mutants in which Tyr-10 and Phe-14 are substituted with Asn and Ser (Vim[Y10N], Vim[F14S] and Vim[Y10N, F14S]), and examined assembly properties in vitro by electron microscopy and viscosity...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Salts
• Share to Amino Acid Motifs
• Share to Amino Acids, Aromatic