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Article

TaqMan-based real-time PCR for genotyping common polymorphisms of haptoglobin (HP1 and HP2).

Clinical chemistry - 1 Nov 2008

Soejima Mikiko, Koda Yoshiro

Abstract excerpt

BACKGROUND: The haptoglobin gene (HP) has 2 common codominant alleles (HP(1) and HP(2)) that account for 3 phenotypes. HP(2) is generated by a 1.7-kb intragenic duplication of HP(1). METHODS: We used the real-time TaqMan PCR system to develop an effective method for HP genotyping that allows us to evaluate the relative number of copies of the HP(2) allele-specific junctional region of the 1.7-kb gene duplication...

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PMID: 18787013
DOI: 10.1373/clinchem.2008.113126

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