Article
A novel internally controlled real-time reverse transcription-PCR assay for HIV-1 RNA targeting the pol integrase genomic region.
Journal of virological methods - 1 Jun 2007
Müller Jens, Eis-Hübinger Anna Maria, Däumer Martin, Kaiser Rolf, Rox Jutta Maria, Gürtler Lutz, Hanfland Peter, Pötzsch Bernd
Abstract excerpt
Given the worldwide increasing spread of HIV-1 genetic variants, it is mandatory that assays used for nucleic acid testing for HIV-1 detect all existing groups and subtypes of HIV-1. In this report the development and evaluation of a quantitative real-time HIV-1 RT-PCR assay that targets a conserved region within the pol integrase domain is described. As an internal control reaction, endogenous...
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