Article
Exploring subdomain cooperativity in T4 lysozyme I: structural and energetic studies of a circular permutant and protein fragment.
Protein science : a publication of the Protein Society - 1 May 2007
Cellitti Jason, Llinas Manuel, Echols Nathaniel, Shank Elizabeth A, Gillespie Blake, Kwon Ester, Crowder Scott M, Dahlquist Frederick W, Alber Tom, Marqusee Susan
Abstract excerpt
Small proteins are generally observed to fold in an apparent two-state manner. Recently, however, more sensitive techniques have demonstrated that even seemingly single-domain proteins are actually made up of smaller subdomains. T4 lysozyme is one such protein. We explored the relative autonomy of its two individual subdomains and their contribution to the overall stability of T4 lysozyme by examining a circular...
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