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Article

LNA incorporated siRNAs exhibit lower off-target effects compared to 2'-OMethoxy in cell phenotypic assays and microarray analysis.

Nucleic acids symposium series (2004) - 1 Jan 2008

Puri Nitin, Wang Xiaohui, Varma Rajeev, Burnett Chris, Beauchamp Lesslie, Batten Diana M, Young Michelle, Sule Vivian, Latham Kathy, Sendera Tim, Echeverri Chris, Sachse Christophe, Magdaleno Susan

Abstract excerpt

Despite the promise of short interfering RNAs (siRNA), contending with off-target is a challenge for RNAi users. To alleviate these problems, we have developed locked nucleic acid (LNA) modified siRNAs and optimized performance using cellular phenotypic assays as well as microarray analysis. During development, we compared LNA and 2'OMethoxy (2'OMe) chemistries placed strategically throughout the siRNA molecule...

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PMID: 18776235
DOI: 10.1093/nass/nrn013

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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