Article
Light controllable siRNAs regulate gene suppression and phenotypes in cells.
Biochimica et biophysica acta - 1 Mar 2006
Nguyen Quan N, Chavli Rajesh V, Marques Joao T, Conrad Peter G, Wang Die, He Weihai, Belisle Barbara E, Zhang Aiguo, Pastor Larry M, Witney Frank R, Morris May, Heitz Frederic, Divita Gilles, Williams Bryan R G, McMaster Gary K
Abstract excerpt
Small interfering RNA (siRNA) is widely recognized as a powerful tool for targeted gene silencing. However, siRNA gene silencing occurs during transfection, limiting its use is in kinetic studies, deciphering toxic and off-target effects and phenotypic assays requiring temporal, and/or spatial regulation. We developed a novel controllable siRNA (csiRNA) that is activated by light. A single photo removable group...
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