Article
Development of an ELA-DRA gene typing method based on pyrosequencing technology.
Tissue antigens - 1 Nov 2008
Díaz S, Echeverría M G, It V, Posik D M, Rogberg-Muñoz A, Pena N L, Peral-García P, Vega-Pla J L, Giovambattista G
Abstract excerpt
The polymorphism of equine lymphocyte antigen (ELA) class II DRA gene had been detected by polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) and reference strand-mediated conformation analysis. These methodologies allowed to identify 11 ELA-DRA exon 2 sequences, three of which are widely distributed among domestic horse breeds. Herein, we describe the development of a...
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