Article
SNP typing of aldehyde dehydrogenase2 gene with Cycleave ICAN.
Molecular and cellular probes - 1 Jan 2000
Mukai Hiroyuki, Takeda Osamu, Usui Kanako, Asada Kiyozo, Kato Ikunoshin
Abstract excerpt
A simple and non-expensive platform is critical to realize on-site SNP typing. In this study we typed an SNP existing at the 487th residue of human aldehyde dehydrogenase2 [wild: Glu (GAA); mutant: Lys (AAA)] using our unique isothermal DNA amplification method, ICAN and cycling probes. Both genotypes were identified by the naked eye using a non-expensive UV transilluminator as well as with real-time PCR...
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